Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Dec;17(6):375-84.
doi: 10.1007/BF00743110.

Effect of trypsin on the kinetic properties of reconstituted beef heart cytochrome c oxidase

U Büge, B Kadenbach

Effect of trypsin on the kinetic properties of reconstituted beef heart cytochrome c oxidase

U Büge et al. J Bioenerg Biomembr. 1985 Dec.

Abstract

Isolated beef heart cytochrome c oxidase was reconstituted in liposomes by the cholate dialysis method with 85% of the binding site for cytochrome c oriented to the outside. Trypsin cleaved specifically subunit VIa and half of subunit IV from the reconstituted enzyme. The kinetic properties of the reconstituted enzyme were changed by trypsin treatment if measured by the spectrophotometric assay but not by the polarographic assay. It is concluded that subunit VIa and/or subunit IV participate in the electron transport activity of cytochrome c oxidase.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Biochem J. 1979 Jul 15;182(1):149-56 - PubMed
    1. Biochemistry. 1980 Sep 2;19(18):4173-8 - PubMed
    1. Hoppe Seylers Z Physiol Chem. 1982 Sep;363(9):1133-40 - PubMed
    1. FEBS Lett. 1984 Jul 9;172(2):189-92 - PubMed
    1. Eur J Biochem. 1980 Apr;105(3):499-507 - PubMed

Publication types