Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jan;202(1):35-41.
doi: 10.1007/BF00330513.

Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12

Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12

T Mogi et al. Mol Gen Genet. 1986 Jan.

Erratum in

  • Mol Gen Genet 1986 Aug;204(2):362

Abstract

Two new mutants of E. coli K12, strains PT9 and PT32 were isolated, that were defective in proline transport. They had no high affinity proline transport activity, but their cytoplasmic membranes retained proline binding activity with altered sensitivity to inhibition by p-chloromercuribenzoate (pCMB). The lesion was mapped at the putP gene, which is located at min 23 on the revised E. coli genetic map (Bachmann 1983) as a composite gene in the proline utilization gene cluster, putP, putC, and putA, arranged in this order. The putC gene was shown to regulate the synthesis of proline dehydrogenase (putA gene product). Hybrid plasmids carrying the put region (Motojima et al. 1979; Wood et al. 1979) were used to construct the physical map of the put region. The possible location of the putP gene in the DNA segment was determined by subcloning the putP gene, genetic complementation, and recombination analyses using several proline transport mutants.

PubMed Disclaimer

References

    1. Can J Biochem. 1979 Oct;57(10):1191-9 - PubMed
    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed
    1. J Bacteriol. 1950 Jul;60(1):17-28 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6255-9 - PubMed
    1. Biochemistry. 1973 Jun 19;12(13):2468-74 - PubMed

Publication types

LinkOut - more resources