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. 2018 Summer;13(3):323-330.
doi: 10.22037/iej.v13i3.19890.

Biodentine for Furcation Perforation Repair: An Animal Study with Histological, Radiographic and Micro-Computed Tomographic Assessment

Miguel Cardoso  1   2 , Maria Dos Anjos Pires  1 , Vitor Correlo  3 , Rui Reis  3 , Manuel Paulo  2 , Carlos Viegas  1   3
Affiliations

Biodentine for Furcation Perforation Repair: An Animal Study with Histological, Radiographic and Micro-Computed Tomographic Assessment

Miguel Cardoso et al. Iran Endod J. 2018 Summer.

Abstract

Introduction: Biodentine has been scarcely studied as a furcation perforation (FP) repair material, mostly by in vitro methodologies. This animal study aimed to compare the histological responses, radiographic, and micro-computed tomographic (micro-CT) outcomes after FP repair with Biodentine or ProRoot MTA (MTA) in dogs' teeth.

Methods and materials: Fifty teeth from five dogs were divided into 4 groups: MTA (n=20, FP repaired with ProRoot MTA), BDT (n=20, FP repaired with Biodentine), PC (n=5, positive control, FP without repair) and NC (n=5, negative control, without perforation). The animals were euthanized after 4 months. Histological assessment included inflammatory cell infiltration, hard tissue resorption, hard tissue repair, and cement repair in the furcation area. Immediate postoperative and 4 months follow-up radiographs were compared for radiolucency in the furcation region. The volume of extruded material was quantified using micro-CT images.

Results: The tested materials showed equivalent radiographic response, together with similar hard tissue resorption and repair but, BDT group showed significantly less inflammation, lower volume of extruded material and higher cement repair than MTA group.

Conclusion: The outcomes of this study, taken together with other favorable results in literature, are highly suggestive that Biodentine is a promising biomaterial to be used for FP repair.

Keywords: Biodentine; Biomaterial; Endodontics; Furcation Perforation; Imaging; Micro-Computed Tomography.

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Conflict of interest statement

‘None declared’.

Figures

Figure 1
Figure 1
Access cavity with furcation perforation (arrow)
Figure 2
Figure 2
Radiographic images. A) BDT group specimen: A1) immediate postoperative, A2) 120 days after furcation perforation repair; B) MTA group specimen: B1) immediate postoperative, B2) 120 days after furcation perforation repair with development of radiolucency (arrow); C) PC group specimen: C1) immediate postoperative with radiolucency (arrow), C2) 120 days after furcation perforation with increase of radiolucency (arrow
Figure 3
Figure 3
Micro-CT volume reconstruction and axial sections. A) Micro-CT 3D model reconstruction representative of extruded material volume; B) Micro-CT axial section of a tooth restored with Biodentine (arrow) in continuity with adjacent bone; C) Micro-CT axial section of a tooth restored with MTA (arrow) in continuity with adjacent bone; D, E, F) Micro-CT axial sections of a tooth restored with Biodentine showing dentine bridge (arrow) formation from coronal to apical (from D to F
Figure 4
Figure 4
Histological images 120 days after furcation perforation repair. A, E) BDT group specimens in different magnifications; B, F, G) MTA group specimens in different magnifications; C) PC group specimen; D) NC group specimen; H) Mineralized bridge over vital pulp in a BDT group specimen. Conventional light microscopy; Hematoxylin-eosin; A, B, C, D) ×4 magnification; E, F) ×20 magnification; G) ×40 magnification; H) ×10 magnification. (Arrow: cementoblasts; *: furcation; **: perforation; ***: pulp chamber; b: bone; bd: Biodentine; cb: cementum bridge; c: cementum; d: dentin; gl: granulation tissue; mb: mineralized bridge; mt: MTA; nc: new cementum; o: odontoblasts; p: vital pulp; pl: periodontal ligament; v: blood vessels
See this image and copyright information in PMC

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