Distribution of Virulence Genes among Salmonella Serotypes Isolated from Pigs in Southern Vietnam

Abstract

This study was carried out to evaluate the prevalence of Salmonella serogroups and serotypes and their virulence gene carriage in pig fecal samples from farms and slaughterhouses in some southern provinces of Vietnam. The presence of Salmonella was assessed based on culture enrichment of the collected samples and biochemical and serological analyses; 27.7% (51) of 184 samples were posititve for Salmonella. Based on the availability of antisera, serogroups were determined for 61% (31) of 51 isolates. Twenty isolates belonging to Salmonella serotypes Typhimurium (10 isolates), Anatum (8 isolates), Senftenberg (7 isolates), Paratyphi B (3 isolates), Paratyphi A (1 isolate), Montevideo (1 isolate), and Saintpaul (1 isolate) were further characterized by a multiplex PCR protocol targeting Salmonella invasion A and virulence plasmid C genes ( invA and spvC, respectively). Individual PCR assays were developed to detect genes for Salmonella enterotoxin ( stn) , Salmonella outer protein B ( sopB), and Salmonella fimbriae ( pef). Various carriage patterns were identified among tested isolates. The invA and sopB genes were found in all isolates, and the stn gene was found in 95% of the isolates. The spvC gene was found in only 5% of the Salmonella Typhimurium isolates. None of the isolates were positive for the pef gene. Among all isolates, the predominant genotypic virulence profile (virulotype) was characterized by the concomitant presence of invA, sopB, and stn in carrier strains. In contrast, two virulotypes comprising either invA, sopB, spvC, and stn or invA and sopB were identified for the Salmonella Typhimurium isolates. Virulotypes made up of multiple virulence genes were predominant in most Salmonella strains tested in this study, indicating that pigs might act as a reservoir for these virulent strains.

Keywords: Pig; Salmonella; Serogroup; Serotype; Virulence genes.