Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10
- PMID: 3009695
- DOI: 10.1099/0022-1317-67-5-957
Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10
Abstract
The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59 163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.
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