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. 1986 Feb;202(2):240-5.
doi: 10.1007/BF00331643.

Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2)

K Kendall, J Cullum

Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2)

K Kendall et al. Mol Gen Genet. 1986 Feb.

Abstract

We identified a DNA element of length about 1 kb that is present in two copies in the chromosome of Streptomyces coelicolor A3(2) and is also present on the plasmid SCP1 which has been carefully defined genetically, but never isolated as extrachromosomal DNA. A copy of the element is close (within 5 kb) of a gene coding for an extracellular agarase in the chromosome of S. coelicolor A3(2) and in an NF strain, in which SCP1 has integrated into the chromosome, the agarase gene has been deleted. The element has properties reminiscent of Insertion Sequences in Escherichia coli, but it is not yet know if it can transpose.

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References

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