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. 1986 May;83(10):3209-12.
doi: 10.1073/pnas.83.10.3209.

Activation of rat c-raf during transfection of hepatocellular carcinoma DNA

F Ishikawa, F Takaku, K Hayashi, M Nagao, T Sugimura

Activation of rat c-raf during transfection of hepatocellular carcinoma DNA

F Ishikawa et al. Proc Natl Acad Sci U S A. 1986 May.

Abstract

Rat c-raf was found to be activated in a transformant obtained with DNA of a hepatocellular carcinoma induced by 2-amino-3-methylimidazo[4,5-f]quinoline. This activated c-raf was cloned in a cosmid vector and actively transforming clones were obtained. Comparison of the restriction maps of this activated c-raf and cloned normal rat c-raf revealed a recombination in the 5'-terminal region of the activated form of this gene. The recombined DNA was shown to be actively transcribed and possibly to form a fused mRNA with c-raf, which is slightly smaller than normal c-raf mRNA. Since this recombination was not detected in the original tumor by Southern blot analysis, it presumably occurred during transfection.

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References

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