Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis

doi:10.1007/s15010-018-1183-8

Review

. 2018 Oct;46(5):687-691.

doi: 10.1007/s15010-018-1183-8.

Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis

Marcin F Osuchowski¹, Alfred Ayala², Soheyl Bahrami³, Michael Bauer⁴, Mihaly Boros⁵, Jean-Marc Cavaillon⁶, Irshad H Chaudry⁷, Craig M Coopersmith⁸, Clifford Deutschman⁹, Susanne Drechsler³, Philip Efron¹⁰, Claes Frostell¹¹, Gerhard Fritsch^{12

13}, Waldemar Gozdzik¹⁴, Judith Hellman¹⁵, Markus Huber-Lang¹⁶, Shigeaki Inoue¹⁷, Sylvia Knapp¹⁸, Andrey V Kozlov³, Claude Libert^{19

20}, John C Marshall²¹, Lyle L Moldawer¹⁰, Peter Radermacher²², Heinz Redl³, Daniel G Remick²³, Mervyn Singer²⁴, Christoph Thiemermann²⁵, Ping Wang²⁶, W Joost Wiersinga²⁷, Xianzhong Xiao²⁸, Basilia Zingarelli²⁹

Affiliations

¹ Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Donaueschingenstrasse 13, 1200, Vienna, Austria. marcin.osuchowski@trauma.lbg.ac.at.
² Rhode Island Hospital and Alpert School of Medicine at Brown University, Providence, RI, USA.
³ Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Donaueschingenstrasse 13, 1200, Vienna, Austria.
⁴ Jena University Hospital, Jena, Germany.
⁵ Institute of Surgical Research, University of Szeged, Szeged, Hungary.
⁶ Institut Pasteur, Paris, France.
⁷ University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA.
⁸ Emory University School of Medicine, Atlanta, GA, USA.
⁹ Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, USA.
¹⁰ University of Florida College of Medicine, Gainesville, FL, USA.
¹¹ Division of Anaesthesia and Intensive Care, Karolinska Institutet, Danderyd Hospital, Stockholm, Sweden.
¹² AUVA Traumacenter, Vienna, Austria.
¹³ Paracelsus Medical University, Salzburg, Austria.
¹⁴ Wroclaw Medical University, Wroclaw, Poland.
¹⁵ School of Medicine, University of California, San Francisco, San Francisco, CA, USA.
¹⁶ Institute of Clinical and Experimental Trauma-Immunology, University Hospital of Ulm, Ulm, Germany.
¹⁷ Kobe University Graduate School of Medicine, Kobe, Japan.
¹⁸ Department of Medicine 1, Medical University Vienna, Vienna, Austria.
¹⁹ Center for Inflammation Research, VIB, Ghent, Belgium.
²⁰ University Ghent, Ghent, Belgium.
²¹ Keenan Research Centre for Biomedical Science, St. Michael's Hospital, University of Toronto, Toronto, Canada.
²² Institute of Anaesthesiological Pathophysiology and Process Development, University Hospital of Ulm, Ulm, Germany.
²³ Boston University School of Medicine, Boston, MA, USA.
²⁴ Bloomsbury Institute of Intensive Care Medicine, University College London, London, UK.
²⁵ The William Harvey Research Institute, Barts and London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
²⁶ Feinstein Institute for Medical Research, Manhasset, NY, USA.
²⁷ Division of Infectious Diseases, and Center for Experimental and Molecular Medicine, the Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
²⁸ Xiangya School of Medicine, Central South University, Chagnsha, Hunan, China.
²⁹ Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.

PMID: 30105433
PMCID: PMC6182493
DOI: 10.1007/s15010-018-1183-8

Review

Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis

Marcin F Osuchowski et al. Infection. 2018 Oct.

. 2018 Oct;46(5):687-691.

doi: 10.1007/s15010-018-1183-8.

Authors

Affiliations

¹ Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Donaueschingenstrasse 13, 1200, Vienna, Austria. marcin.osuchowski@trauma.lbg.ac.at.
² Rhode Island Hospital and Alpert School of Medicine at Brown University, Providence, RI, USA.
³ Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Donaueschingenstrasse 13, 1200, Vienna, Austria.
⁴ Jena University Hospital, Jena, Germany.
⁵ Institute of Surgical Research, University of Szeged, Szeged, Hungary.
⁶ Institut Pasteur, Paris, France.
⁷ University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA.
⁸ Emory University School of Medicine, Atlanta, GA, USA.
⁹ Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, USA.
¹⁰ University of Florida College of Medicine, Gainesville, FL, USA.
¹¹ Division of Anaesthesia and Intensive Care, Karolinska Institutet, Danderyd Hospital, Stockholm, Sweden.
¹² AUVA Traumacenter, Vienna, Austria.
¹³ Paracelsus Medical University, Salzburg, Austria.
¹⁴ Wroclaw Medical University, Wroclaw, Poland.
¹⁵ School of Medicine, University of California, San Francisco, San Francisco, CA, USA.
¹⁶ Institute of Clinical and Experimental Trauma-Immunology, University Hospital of Ulm, Ulm, Germany.
¹⁷ Kobe University Graduate School of Medicine, Kobe, Japan.
¹⁸ Department of Medicine 1, Medical University Vienna, Vienna, Austria.
¹⁹ Center for Inflammation Research, VIB, Ghent, Belgium.
²⁰ University Ghent, Ghent, Belgium.
²¹ Keenan Research Centre for Biomedical Science, St. Michael's Hospital, University of Toronto, Toronto, Canada.
²² Institute of Anaesthesiological Pathophysiology and Process Development, University Hospital of Ulm, Ulm, Germany.
²³ Boston University School of Medicine, Boston, MA, USA.
²⁴ Bloomsbury Institute of Intensive Care Medicine, University College London, London, UK.
²⁵ The William Harvey Research Institute, Barts and London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
²⁶ Feinstein Institute for Medical Research, Manhasset, NY, USA.
²⁷ Division of Infectious Diseases, and Center for Experimental and Molecular Medicine, the Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
²⁸ Xiangya School of Medicine, Central South University, Chagnsha, Hunan, China.
²⁹ Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.

PMID: 30105433
PMCID: PMC6182493
DOI: 10.1007/s15010-018-1183-8

Erratum in

Correction to: Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis.
Osuchowski MF, Ayala A, Bahrami S, Bauer M, Boros M, Cavaillon JM, Chaudry IH, Coopersmith CM, Deutschman C, Drechsler S, Efron P, Frostell C, Fritsch G, Gozdzik W, Hellman J, Huber-Lang M, Inoue S, Knapp S, Kozlov AV, Libert C, Marshall JC, Moldawer LL, Radermacher P, Redl H, Remick DG, Singer M, Thiemermann C, Wang P, Wiersinga WJ, Xiao X, Zingarelli B. Osuchowski MF, et al. Infection. 2018 Oct;46(5):745-747. doi: 10.1007/s15010-018-1197-2. Infection. 2018. PMID: 30225655 Free PMC article.

Abstract

Purpose: Pre-clinical animal studies precede the majority of clinical trials. While the clinical sepsis definitions and recommended treatments are regularly updated, a systematic review of pre-clinical models of sepsis has not been done and clear modeling guidelines are lacking. To address this deficit, a Wiggers-Bernard Conference on pre-clinical sepsis modeling was held in Vienna in May, 2017. The conference goal was to identify limitations of pre-clinical sepsis models and to propose a set of guidelines, defined as the "Minimum Quality Threshold in Pre-Clinical Sepsis Studies" (MQTiPSS), to enhance translational value of these models.

Methods: 31 experts from 13 countries participated and were divided into 6 thematic Working Groups (WG): (1) Study Design, (2) Humane modeling, (3) Infection types, (4) Organ failure/dysfunction, (5) Fluid resuscitation and (6) Antimicrobial therapy endpoints. As basis for the MQTiPSS discussions, the participants conducted a literature review of the 260 most highly cited scientific articles on sepsis models (2002-2013).

Results: Overall, the participants reached consensus on 29 points; 20 at "recommendation" (R) and 9 at "consideration" (C) strength. This Executive Summary provides a synopsis of the MQTiPSS consensus (Tables 1, 2 and 3).

Conclusions: We believe that these recommendations and considerations will serve to bring a level of standardization to pre-clinical models of sepsis and ultimately improve translation of pre-clinical findings. These guideline points are proposed as "best practices" that should be implemented for animal sepsis models. In order to encourage its wide dissemination, this article is freely accessible in Shock, Infection and Intensive Care Medicine Experimental.

Keywords: Antimicrobial therapy; Experiment; Fluid resuscitation; Guidelines; Humane modeling; Infection types; Organ dysfunction; Study design.

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Conflict of interest statement

Claes Frostell controls the Claes Frostell Research & Consulting AB company that participated in supporting several clinical and experimental studies. Other authors do not declare any conflict of interest.

Comment in

The Response to the Letter to the Editor Titled: "Is Triple Self-plagiarism "OK" If Only Made Transparent?" by Volker R Jacobs, MD, MBA.
Osuchowski MF, Hellman J, Huber-Lang M, Libert C, Remick DG, Thiemermann C, Zingarelli B. Osuchowski MF, et al. Shock. 2019 Jan;51(1):140-141. doi: 10.1097/SHK.0000000000001294. Shock. 2019. PMID: 30475325 No abstract available.
Is Triple Self-Plagiarism "OK" If Only Made Transparent?
Jacobs VR. Jacobs VR. Shock. 2019 Jan;51(1):139. doi: 10.1097/SHK.0000000000001293. Shock. 2019. PMID: 30475326 No abstract available.
Comment From the Editorial Office on "Is Triple Self-Plagiarism "OK" if Only Made Transparent?".
Clemens MG. Clemens MG. Shock. 2019 Jan;51(1):142. doi: 10.1097/SHK.0000000000001295. Shock. 2019. PMID: 30475327 No abstract available.

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References

1. Lamberg L. Researchers urged to tell public how animal studies benefit human health. JAMA. 1999;282:619–621. doi: 10.1001/jama.282.7.619. - DOI - PubMed
1. Van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O’Collins V, Macleod MR. Can animal models of disease reliably inform human studies? PLoS Med. 2010;7:e1000245. doi: 10.1371/journal.pmed.1000245. - DOI - PMC - PubMed
1. Stortz JA, Raymond SL, Mira JC, Moldawer LL, Mohr AM, Efron PA. Murine models of sepsis and trauma: can we bridge the gap? ILAR. 2017;J 58:90–105. doi: 10.1093/ilar/ilx007. - DOI - PMC - PubMed
1. Van der Poll T. Preclinical sepsis models. Surg Infect (Larchmt) 2012;13:287–292. doi: 10.1089/sur.2012.105. - DOI - PubMed
1. Coen D. Oxford TB vaccine study calls into question selective use of animal data. BMJ. 2012;360:j5845.

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[1] Lamberg L. Researchers urged to tell public how animal studies benefit human health. JAMA. 1999;282:619–621. doi: 10.1001/jama.282.7.619. - DOI - PubMed

[2] Lamberg L. Researchers urged to tell public how animal studies benefit human health. JAMA. 1999;282:619–621. doi: 10.1001/jama.282.7.619. - DOI - PubMed

[3] Van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O’Collins V, Macleod MR. Can animal models of disease reliably inform human studies? PLoS Med. 2010;7:e1000245. doi: 10.1371/journal.pmed.1000245. - DOI - PMC - PubMed

[4] Van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O’Collins V, Macleod MR. Can animal models of disease reliably inform human studies? PLoS Med. 2010;7:e1000245. doi: 10.1371/journal.pmed.1000245. - DOI - PMC - PubMed

[5] Stortz JA, Raymond SL, Mira JC, Moldawer LL, Mohr AM, Efron PA. Murine models of sepsis and trauma: can we bridge the gap? ILAR. 2017;J 58:90–105. doi: 10.1093/ilar/ilx007. - DOI - PMC - PubMed

[6] Stortz JA, Raymond SL, Mira JC, Moldawer LL, Mohr AM, Efron PA. Murine models of sepsis and trauma: can we bridge the gap? ILAR. 2017;J 58:90–105. doi: 10.1093/ilar/ilx007. - DOI - PMC - PubMed

[7] Van der Poll T. Preclinical sepsis models. Surg Infect (Larchmt) 2012;13:287–292. doi: 10.1089/sur.2012.105. - DOI - PubMed

[8] Van der Poll T. Preclinical sepsis models. Surg Infect (Larchmt) 2012;13:287–292. doi: 10.1089/sur.2012.105. - DOI - PubMed

[9] Coen D. Oxford TB vaccine study calls into question selective use of animal data. BMJ. 2012;360:j5845.

[10] Coen D. Oxford TB vaccine study calls into question selective use of animal data. BMJ. 2012;360:j5845.

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Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis

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Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis

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