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. 2018 Sep;62(9):607-611.
doi: 10.1111/1348-0421.12641.

Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis

Satoshi Higashide  1 , Otomi Cho  1 , Yuko Matsuda  1   2 , Daiki Ogishima  2 , Sanae Kurakado  1 , Takashi Sugita  1
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Free article

Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis

Satoshi Higashide et al. Microbiol Immunol. 2018 Sep.
Free article

Abstract

The aim of this study was to develop a method for the rapid detection of Gardnerella vaginalis, which is proposed to play a key role in the pathogenesis of bacterial vaginosis. Specific loop-mediated isothermal amplification (LAMP) primers were designed and used to detect target DNA within 45 min under isothermal conditions. Comparative screening indicated that the LAMP assay is superior to PCR in terms of rapidity, and is equivalent in sensitivity and specificity. This LAMP assay can be used for rapid screening and detection of G. vaginalis in vaginal samples; the limit of detection is 10 fg DNA.

Keywords: Gardnerella vaginalis; bacterial vaginosis; loop-mediated isothermal amplification; rapid detection.

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