doi: 10.1016/0378-1119(86)90111-3.
Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens
- PMID: 3011602
- DOI: 10.1016/0378-1119(86)90111-3
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Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens
Gene.
1986.
Abstract
Two cDNA clones encoding two major human trypsinogen isozymes were isolated from a human pancreatic cDNA library. The deduced amino acid (aa) sequences of the two trypsinogen precursors are found to have 89% sequence homology, and have the same number of aa (247), including 15 aa for a signal peptide and 8 aa for an activation peptide. Southern blot analysis of human genomic DNA using the cloned cDNA as a probe, revealed that the human trypsinogen genes constitute a multigene family of more than ten genes.
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