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. 2018 Aug;16(2):1318-1322.
doi: 10.3892/etm.2018.6278. Epub 2018 Jun 8.

Trimetazidine combined with berberine on endothelial function of patients with coronary heart disease combined with primary hypertension

Hui Zhang  1 , Hongyu Niu  1 , Xiangzhi Yuan  1 , Jing Chang  1 , Xiaojuan Wang  1
Affiliations

Trimetazidine combined with berberine on endothelial function of patients with coronary heart disease combined with primary hypertension

Hui Zhang et al. Exp Ther Med. 2018 Aug.

Abstract

Effects of trimetazidine combined with berberine on endothelial function of patients with coronary heart disease combined with primary hypertension (CCP) were investigated. A total of 68 patients with CCP were selected from July 2014 to August 2016 to serve as observation group. At the same time, 68 healthy people were also selected to serve as control group (physiological saline). Expression of endothelial nitric oxide synthase (eNOS) mRNA in the blood samples of the observation and control groups before and after treatment was determined by RT-PCR. Levels of NO in the plasma of the observation and control groups before and after treatment were measured by nitric acid reductase method. Brachial artery flow-mediated vasodilation (FMD) of observation and control groups was detected by brachial artery ultrasonography before and after treatment. Before treatment, expression level of eNOS mRNA in blood of the observation group was significantly lower than that of the control group (P<0.05). After treatment, expression level of eNOS mRNA was significantly increased (P<0.05). Plasma NO content 41.06±3.63 mol/l in blood of the observation group was significantly lower than that of the control group 53.28±3.09 mol/l (P<0.05). After treatment with trimetazidine and berberine, level of NO 50.75±2.75 mol/l was significantly increased compared with the level before treatment (P<0.05). FMD value (5.03±0.95) was significantly lower in observation group than that in control group (16.04±1.63) (P<0.05). After treatment with trimetazidine and berberine, FMD value (14.02±2.39) was significantly increased compared with the level before treatment (P<0.05). The results suggested that the combination of trimetazidine and berberine can increase the content of NO in blood and promote endothelium-dependent dilation function of brachial arteries, which is helpful in the treatment of CCP.

Keywords: berberine; coronary artery disease; eNOS gene; primary hypertension; trimetazidine.

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Figures

Figure 1.
Figure 1.
Relative expression level of endothelial nitric oxide synthase (eNOS) mRNA in CCP patients and healthy people. Results of quantitative RT-PCR showed that, compared with control group, expression level of eNOS mRNA in CCP patients was significantly decreased (P=0.028<0.05).
Figure 2.
Figure 2.
Relative expression levels of endothelial nitric oxide synthase (eNOS) mRNA in CCP patients before and after treatment. Treatment with trimetazidine and berberine significantly increased expression level of eNOS mRNA in CCP patients (P=0.032<0.05).
Figure 3.
Figure 3.
Nitric oxide (NO) content in blood of CCP patients and healthy people. NO content 53.28±3.09 mol/l in blood of the control group was significantly lower than that of the observation group 41.06±3.63 mol/l (P=0.027<0.05, F=3985.631, t=0.1873).
Figure 4.
Figure 4.
Nitric oxide (NO) content in blood of CCP patients before and after treatment (P=0.021<0.05).
Figure 5.
Figure 5.
Determination of flow-mediated vasodilation (FMD) value in CCP patients and healthy people (P<0.05).
Figure 6.
Figure 6.
Flow-mediated vasodilation (FMD) in patients with CCP before and after treatment (P<0.05).
See this image and copyright information in PMC

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