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. 2018 Apr-Jun;10(2):93-96.

ATP Reduces the Entry of Calcium Ions into the Nerve Ending by Blocking L-type Calcium Channels

E F Khaziev  1   2   3 , D V Samigullin  1   2   3 , A N Tsentsevitsky  1   2 , E A Bukharaeva  1   2 , E E Nikolsky  1   2
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ATP Reduces the Entry of Calcium Ions into the Nerve Ending by Blocking L-type Calcium Channels

E F Khaziev et al. Acta Naturae. 2018 Apr-Jun.

Abstract

At neuromuscular junctions, ATP inhibits both the evoked and spontaneous acetylcholine release and inward calcium current operating via presynaptic P2Y receptors. It was shown in the experiments with the frog neuromuscular synapse using specific calcium-sensitive dye Oregon Green Bapta 1 that exogenous ATP reduces the amplitude of calcium transient, which reflects the changes in the entry of calcium ions in response to the nerve pulse. The depressing effect of ATP on the transient was prevented by suramin, the blocker of P2 receptors. Nitrendipine, a specific blocker of L-type calcium channels, per se decreased the calcium transient amplitude and significantly attenuated the effect of ATP on the calcium signal. Contrariwise, the preliminary application of ATP to the neuromuscular junction completely eliminated the depressing effect of nitrendipine on the calcium response. The obtained data suggest that an essential component in the inhibitory action of ATP on the calcium transient amplitude is provided by reduction of the entry of calcium ions into a frog nerve ending via L-type voltage-gated calcium channels.

Keywords: ATP; calcium channels; calcium transient; neuromuscular junction.

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Figures

Fig. 1
Fig. 1
ATP reduces the amplitude of calcium transient operating via P2 receptors. A – The effect of 10 μM ATP on Ca2+ transient. B – The absence of ATP effect on transient in the presence of suramin. A, B – the averaging of 60 fluorescence signals. C – Effect of ATP (10 and 100 μM), suramin (Sur), and ATP in the presence of suramin on the amplitude of Ca2+ transient. The averaged changes in the Ca2+ transient amplitude for ATP and suramin (Sur) are expressed as a percentage of related calcium signal amplitudes under control conditions. In the case of combined action of suramin and ATP (Sur+ATP), the amplitude of Ca2+ transient under suramin was taken as 100%. * p < 0.05
Fig. 2
Fig. 2
ATP-induced reduction in the calcium transient amplitude is associated with blockade of presynaptic L-type voltage-gated calcium channels. The depressing effect of ATP is attenuated after the preliminary blocking of L-type calcium channels by nitrendipine (Nitr+ATP). Nitrendipine per se decreases the amplitude of Ca2+ transient (Nitr). Nitrendipine does not alter the amplitude of transient under conditions when P2 receptors are activated by ATP (ATP+Nitr). * p < 0.05
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