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. 1986 Jun;166(3):1118-22.
doi: 10.1128/jb.166.3.1118-1122.1986.

Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene

T Takano, M Fukuda, M Monma, S Kobayashi, K Kainuma, K Yamane

Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene

T Takano et al. J Bacteriol. 1986 Jun.

Abstract

The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.

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References

    1. Virology. 1970 Mar;40(3):734-44 - PubMed
    1. Biochemistry. 1968 Jan;7(1):114-20 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Carbohydr Res. 1978 Mar;61:229-38 - PubMed
    1. Nucleic Acids Res. 1978 Jul;5(7):2321-32 - PubMed

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