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. 2018 Jul 9:2018:7646104.
doi: 10.1155/2018/7646104. eCollection 2018.

PPAR- γ Promotes Hematoma Clearance through Haptoglobin-Hemoglobin-CD163 in a Rat Model of Intracerebral Hemorrhage

Gaiqing Wang  1   2 , Tong Li  1 , Shu-Na Duan  1 , Liang Dong  1 , Xin-Gang Sun  2 , Fang Xue  2
Affiliations

PPAR- γ Promotes Hematoma Clearance through Haptoglobin-Hemoglobin-CD163 in a Rat Model of Intracerebral Hemorrhage

Gaiqing Wang et al. Behav Neurol. .

Abstract

Background and purpose: PPAR-γ is a transcriptional factor which is associated with promoting hematoma clearance and reducing neurological dysfunction after intracerebral hemorrhage (ICH). Haptoglobin- (Hp-) hemoglobin- (Hb-) CD163 acts as a main pathway to Hb scavenging after ICH. The effect of PPAR-γ on the Hp-Hb-CD163 signaling pathway has not been reported. We hypothesized that PPAR-γ might protect against ICH-induced neuronal injury via activating the Hp-Hb-CD163 pathway in a rat ICH model.

Methods: 107 Sprague-Dawley rats were used in this research. They were randomly allocated to 4 groups as follows: sham group, vehicle group, monascin-treated group, and Glivec-treated group. Animals were euthanized at 3 days after the model was established successfully. We observed the effects of PPAR-γ on the brain water content, hemoglobin levels, and the expressions of CD163 and Hp in Western blot and real-ime PCR; meanwhile, we measured hematoma volumes and edema areas by MRI scanning.

Result: The results showed that PPAR-γ agonist significantly reduced hematoma volume, brain edema, and hemoglobin after ICH. It also enhanced CD163 and Hp expression while PPAR-γ antagonist had the opposite effects.

Conclusions: PPAR-γ promotes hematoma clearance and plays a protective role through the Hp-Hb-CD163 pathway in a rat collagenase infusion ICH model.

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Figures

Figure 1
Figure 1
Effect of PPAR-γ on brain water content associated with ICH 3 days after surgery (∗p < 0.05 versus sham; #p < 0.05 versus vehicle).
Figure 2
Figure 2
Effect of PPAR-γ on hemoglobin levels associated with ICH 3 days after surgery (∗p < 0.05 versus sham; #p < 0.05 versus vehicle).
Figure 3
Figure 3
Effect of Glivec and monascin on PPAR-γ, haptoglobin, and CD163 associated with ICH 3 days after surgery. Representative images are shown of Western blot assay (a–d) and real-time PCR (e and f) for PPAR-γ, haptoglobin, and CD163 levels within ipsilateral brain tissues. One-way ANOVA followed by Tukey's tests was used. (∗p < 0.05 versus sham; #p < 0.05 versus vehicle).
Figure 4
Figure 4
Effect of PPAR-γ on hematoma volume (a–c) and brain edema (a, d, and e) associated with ICH 3 days after surgery. Representative images are shown of T2∗WI (a and b), SWI (a and c) for hematoma volume, T2WI (a and d), and T2 Flair (a and e) for brain edema within ipsilateral brain tissues. One-way ANOVA followed by Tukey's tests were used (#p < 0.05 versus vehicle).
See this image and copyright information in PMC

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