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Comment
. 2018 Jul;10(Suppl 18):S2221-S2222.
doi: 10.21037/jtd.2018.06.49.

Interactome analysis reveals MALAT1 binding with DBC1-author's reply

Ruibing Chen  1 , Yun Liu  1 , Hao Zhuang  1   2 , Xinran Zhang  1 , Xiaowen Shao  1 , Yamei Dang  1 , Fei Wu  1 , Yongmei Li  1 , Ning Zhang  3
Affiliations
Comment

Interactome analysis reveals MALAT1 binding with DBC1-author's reply

Ruibing Chen et al. J Thorac Dis. 2018 Jul.
No abstract available

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Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Localization of MALAT1 in HepG2 cells. To detect MALAT1 RNA, cells were rinsed briefly in PBS and then fixed in 4% formaldehyde for 10 min at RT. Further, the cells were permeabilized in PBS containing 0.2% Triton X-100 for 10 min at RT; washed with PBS 3×10 min and rinsed once in 2× SSC prior to hybridization. 6,918–8,441 fragment of MALAT1 antisense was transcribed in vitro, labeled with biotin and used as probes. Hybridization was carried out in a moist chamber at 42 °C for 2 hours. After incubated with biotin-labeled Alexa Fluor 546-conjugated secondary antibody, fluorescence mages were acquired using confocal laser scanning microscopy (Olympus FV1000). MALAT1, red color. MALAT1, metastasis-associated lung adenocarcinoma transcript 1.
See this image and copyright information in PMC

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