Zika Virus Seropositivity in 1-4-Year-Old Children, Indonesia, 2014

Abstract

We assessed Zika virus seroprevalence among healthy 1-4-year-old children using a serum sample collection assembled in 2014 representing 30 urban sites across Indonesia. Of 662 samples, 9.1% were Zika virus seropositive, suggesting widespread recent Zika virus transmission and immunity. Larger studies are needed to better determine endemicity in Indonesia.

Keywords: Indonesia; PRNT; Zika virus; flavivirus; plaque-reduction neutralization; prevalence; serology; seroprevalence; vector-borne infections; viruses.

Figure

Geographic distribution of Zika virus–seropositive 1−4-year-old children, Indonesia, October–November, 2014. The values listed for each province indicate the percentage of serum samples confirmed Zika virus seropositive (percentage serum samples suspected to be Zika virus seropositive). Samples suspected to be Zika virus positive were those that were positive on initial Zika virus PRNT₉₀ (plaque reduction neutralization test with neutralization defined as >90% reduction in challenge virus PFUs) screening when using a 1:10 serum sample dilution. Serum samples confirmed as Zika virus seropositive were those that neutralized Zika virus only or had a PRNT₉₀ titer for Zika virus that was >4-fold higher than the PRNT₉₀ titer for any DENV.