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. 2018 Nov;36(10):946-949.
doi: 10.1038/nbt.4198. Epub 2018 Aug 20.

Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion

Xiao Wang  1   2   3 , Jianan Li  1   2   3 , Ying Wang  4 , Bei Yang  5 , Jia Wei  4 , Jing Wu  1 , Ruixuan Wang  1 , Xingxu Huang  1 , Jia Chen  1 , Li Yang  4
Affiliations

Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion

Xiao Wang et al. Nat Biotechnol. 2018 Nov.

Abstract

Base editors (BEs) enable the generation of targeted single-nucleotide mutations, but currently used rat APOBEC1-based BEs are relatively inefficient in editing cytosines in highly methylated regions or in GpC contexts. By screening a variety of APOBEC and AID deaminases, we show that human APOBEC3A-conjugated BEs and versions we engineered to have narrower editing windows can mediate efficient C-to-T base editing in regions with high methylation levels and GpC dinucleotide content.

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Comment in

  • Better base editors.
    Rusk N. Rusk N. Nat Methods. 2018 Oct;15(10):763. doi: 10.1038/s41592-018-0154-4. Nat Methods. 2018. PMID: 30275576 No abstract available.

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