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. 2018 Nov;37(11):2165-2169.
doi: 10.1007/s10096-018-3356-x. Epub 2018 Aug 21.

Dissemination of blaOXA-370 is mediated by IncX plasmids and the Tn6435 transposon

Cibele M Magagnin  1   2   3 , Juliana C Campos  4 , Darlan A da Rocha  4 , Suely C F Sampaio  5 , Franciéli P Rozáles  6 , Afonso L Barth  6 , Alexandre P Zavascki  7   8 , Jorge L M Sampaio  9   4
Affiliations

Dissemination of blaOXA-370 is mediated by IncX plasmids and the Tn6435 transposon

Cibele M Magagnin et al. Eur J Clin Microbiol Infect Dis. 2018 Nov.

Abstract

In Enterobacteriaceae, the blaOXA-48-like genes have been identified on plasmids in different regions of the world. The OXA-370 is a plasmid-encoded OXA-48-like enzyme reported in two distinct regions of Brazil. Recently, we demonstrate that the blaOXA-370 gene is disseminated among several Enterobacteriaceae species and clones, indicating a high potential for dissemination. In this work, we described for the first time the complete nucleotide sequence of six plasmids harboring the blaOXA-370 gene. Complete DNA sequencing using the Illumina platform and annotation of the plasmids showed that they belonged to incompatibility groups IncX and had in average 70 kbp. The blaOXA-370 gene is located in a composite transposon containing four genes encoding transposases, named Tn6435. In this study, highly similar plasmids were detected in different Enterobacteriaceae genera.

Keywords: Brazil; Enterobacteriaceae; OXA-370; Plasmids; Tn6435.

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References

    1. Bush K, Jacoby GA (2010) Updated functional classification of β-lactamases. Antimicrob Agents Chemother 54:969–976 - DOI
    1. Sampaio JL, Ribeiro VB, Campos JC, Rozales FP, Magagnin CM, Falci DR, da Silva RC, Dalarosa MG, Luz DI, Vieira FJ, Antochevis LC, Barth AL, Zavascki AP (2014) Detection of OXA-370, an OXA-48-related class D β-lactamase, in Enterobacter hormaechei from Brazil. Antimicrob Agents Chemother 58:3566–3567 - DOI - PubMed - PMC
    1. Pereira PS, Borghi M, de Araujo CF, Aires CA, Oliveira JC, Asensi MD, Carvalho-Assef AP (2015) Clonal dissemination of OXA-370-producing Klebsiella pneumoniae in Rio de Janeiro, Brazil. Antimicrob Agents Chemother 59:4453–4456 - DOI - PubMed - PMC
    1. Magagnin CM, Rozales FP, Antochevis L, Nunes LS, Martins AS, Barth AL, Sampaio JM, Zavascki AP (2017) Dissemination of bla OXA-370 gene among several Enterobacteriaceae species in Brazil. Eur J Clin Microbiol Infect Dis 36:1907–1910 - DOI
    1. Maiwald M (2004) Broad-range PCR for detection and identification of bacteria. In: Persing DH (ed) Molecular microbiology diagnostic principles and practice, 2rd edn. American Society for Microbiology, Washington, DC, pp 379–390

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