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. 1986;244(2):265-70.
doi: 10.1007/BF00219201.

Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

N Yoshimura, T Murachi, R Heath, J Kay, B Jasani, G R Newman

Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

N Yoshimura et al. Cell Tissue Res. 1986.

Abstract

By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

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