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. 2018 Aug;34(4):335-340.
doi: 10.5423/PPJ.NT.11.2017.0234. Epub 2018 Aug 1.

Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores

Masahiko Tamaki  1 , Fumiyuki Kobayashi  2 , Hiromi Ikeura  3 , Michio Sato  1
Affiliations

Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores

Masahiko Tamaki et al. Plant Pathol J. 2018 Aug.

Abstract

To investigate the difference in the disinfectant efficiency of ozone microbubbles (O3MB) and ozone millibubbles (O3MMB), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of O3MB on F. oxysporum f. sp. melonis spores was greater than that of O3MMB. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was caused by O3MB and O3MMB, and damage to the spore surfaces by O3MB occurred sooner than that by O3MMB. On observation with TEM, it was furthermore confirmed that F. oxysporum f. sp. melonis spores treated with O3MB induced wavy deformation of cell membrane and the intracellular change different from that with O3MMB. Therefore, the greater disinfection efficiency of O3MB was suggested to be caused due to the function of the MB in addition to the oxidative power of O3.

Keywords: Fusarium oxysporum f. sp. melonis spores; disinfection; ozone microbubbles.

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Figures

Fig. 1
Fig. 1
Disinfection of F. oxysporum f. sp. melonis spores by O3MB and O3MMB. The data presented was the mean of duplicate.
Fig. 2
Fig. 2
SEM images of F. oxysporum f. sp. melonis spores. (A) After treatment with O3MMB for 30 s, (B) After treatment with O3MMB for 60 s, (C) After treatment with O3MB for 30 s, (D) After treatment with O3MB for 60 s, (E) Non-treated.
Fig. 3
Fig. 3
The widths of F. oxysporum f. sp. melonis spores in SEM images. NT: Non-treated. The results indicate the means with standard deviation of 9 spores.
Fig. 4
Fig. 4
TEM images of F. oxysporum f. sp. melonis spores. Top: Non-treated, Middle: After treatment with O3MMB for 180 s, Bottom: After treatment with O3MB for 180 s.
See this image and copyright information in PMC

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