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. 1986 Jul;83(14):5057-61.
doi: 10.1073/pnas.83.14.5057.

Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs

S S Su, P Modrich

Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs

S S Su et al. Proc Natl Acad Sci U S A. 1986 Jul.

Abstract

The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein specifically binds to DNA regions containing a single base-pair mismatch. The protein displayed variable affinity for the limited set of mismatches tested (G-T greater than G-A approximately equal to A-C greater than T-C).

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