doi: 10.1073/pnas.83.14.5057.
Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs
- PMID: 3014530
- PMCID: PMC323889
- DOI: 10.1073/pnas.83.14.5057
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Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs
Proc Natl Acad Sci U S A.
1986 Jul.
Abstract
The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein specifically binds to DNA regions containing a single base-pair mismatch. The protein displayed variable affinity for the limited set of mismatches tested (G-T greater than G-A approximately equal to A-C greater than T-C).
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