Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jul;52(1):206-8.
doi: 10.1128/aem.52.1.206-208.1986.

Mobilization of Tn5 insertions from Rhizobium fredii by pJB3JI

P M Thomas, L D Kuykendall, J S Angle

Mobilization of Tn5 insertions from Rhizobium fredii by pJB3JI

P M Thomas et al. Appl Environ Microbiol. 1986 Jul.

Abstract

Techniques for in vivo cloning were used with the fast-growing nitrogen-fixing soybean microsymbiont R. fredii USDA 191. Selection for transfer of Tn5 insertions from R. fredii USDA 191 containing the gene-mobilizing plasmid pJB3JI provided recombinants at up to 400 times the background mutation level. These techniques may be useful for future genetic analysis of R. fredii.

PubMed Disclaimer

References

    1. J Bacteriol. 1982 Nov;152(2):928-31 - PubMed
    1. Appl Environ Microbiol. 1979 May;37(5):862-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 May;81(10):3093-7 - PubMed
    1. Appl Environ Microbiol. 1983 Oct;46(4):906-11 - PubMed
    1. J Gen Microbiol. 1974 Sep;84(1):188-98 - PubMed

Publication types

LinkOut - more resources