Antimicrobial photodynamic therapy of Lactobacillus acidophilus by indocyanine green and 810-nm diode laser

Abstract

This study investigated the efficacy of photodynamic therapy (PDT) using EmunDo as a photosensitizer against Lactobacillus acidophilus. A gallium aluminum arsenide diode laser was used in this experiment (810 nm, CW). Standard suspensions of Lactobacillus acidophilus were divided into six groups by treatment: 1) EmunDo, 2) diode laser (100 mW, 90 s), 3) diode laser (300 mW, 30 s); 4) EmunDo + diode laser (100 mW, 90 s), 5) EmunDo + diode laser (300 mW, 30 s), 6) control (no treatment). Bacterial suspensions from each group were subcultured onto the surface of MRS agar plates immediately and 24 h after treatment, and the viable microorganisms of Lactobacillus acidophilus were counted. The data were analyzed by ANOVA and student's t-test at p < 0.05. There was a significant between-group difference in the number of Lactobacillus acidophilus colonies in cell cultures obtained at 24 h after treatment (p < 0.001). The viable counts were significantly lower in EmunDo and both PDT groups, as compared to the other groups (p < 0.05). In the control and laser-irradiated groups, the number of colonies increased significantly at 24 h compared to the immediately after treatment (p < 0.05), whereas in both PDT groups, the number of colonies showed a significant reduction after 24 h of therapy (p < 0.05). Under the conditions used in this study, L. acidophilus colonies were susceptible to PDT after sensitization with EmunDo and exposure to diode laser. These findings imply that PDT is capable to reduce cariogenic bacteria, potentially leading to more conservative cavity preparation.

Keywords: Antibacterial; Antimicrobial photodynamic therapy; Cariogenic bacteria; EmunDo; Indocyanine green; Lactobacillus acidophilus; Laser; Photodynamic therapy; Photosensitizer.