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. 1986 Aug;167(2):711-2.
doi: 10.1128/jb.167.2.711-712.1986.

Transposition of the gram-positive transposon Tn917 in Escherichia coli

H K Kuramitsu, M J Casadaban

Transposition of the gram-positive transposon Tn917 in Escherichia coli

H K Kuramitsu et al. J Bacteriol. 1986 Aug.

Abstract

The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-negative bacterium at a relatively high frequency.

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