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. 2018 Aug 7:2018:8207098.
doi: 10.1155/2018/8207098. eCollection 2018.

Assessment of Extrapulmonary Tuberculosis Using Gene Xpert MTB/RIF Assay and Fluorescent Microscopy and Its Risk Factors at Dessie Referral Hospital, Northeast Ethiopia

Yeshi Metaferia  1 , Abdurahaman Seid  1 , Genet Mola Fenta  1 , Daniel Gebretsadik  1
Affiliations

Assessment of Extrapulmonary Tuberculosis Using Gene Xpert MTB/RIF Assay and Fluorescent Microscopy and Its Risk Factors at Dessie Referral Hospital, Northeast Ethiopia

Yeshi Metaferia et al. Biomed Res Int. .

Abstract

Background: Tuberculosis is a major public health problem and extrapulmonary tuberculosis (EPTB) accounts for a significant proportion of tuberculosis cases worldwide.

Objective: To determine the magnitude of EPTB, associated risk factors, and agreement of diagnostic techniques at Dessie Referral Hospital.

Methods: A cross-sectional study was conducted on consecutive presumptive EPTB cases from March 1 to June 30, 2017. Sociodemographic characteristics and other variables were collected using a structured questionnaire. Clinical specimens were collected and processed using fluorescent microscopy and Gene Xpert assay. Data was analyzed using SPSS version 20. Chi-square test and logistic regression were done and a P value of ≤0.05 was taken as statistically significant.

Results: From a total of 353 presumptive EPTB cases the overall prevalence of Gene Xpert assay and smear confirmed patients was 8.8% and 2.5%, respectively. Tuberculosis lymphadenitis was the predominant (33.3%) type followed by pleural (11.9%) and peritoneal (6.7%) tuberculosis. Previous history of pulmonary tuberculosis was significantly associated with extrapulmonary infection (AOR:2.8; 95%CI: 1.05-7.54; p=0.04); however, other variables such as age, residence, sex, marital status, occupation, level of education, and monthly income did not show any association.

Conclusion: High proportions (71%) of Gene Xpert assay confirmed EPTB patients were smear-negative. Sensitivity of microscopy should be enhanced in resource limited countries like Ethiopia where Gene Xpert machine is not easily accessible.

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References

    1. Global tuberculosis report. http://www.who.int/tb/publications/global_report/en/, WHO 2017.
    1. World Health Organization Global Tuberculosis Report 2014, WHO, Geneva, Switzerland. http://apps.who.int/iris/bitstream/handle/10665/137094/9789241564809_eng..., 2014.
    1. Fitzgerald D. W., Sterling T. R., Haas D. W. Mycobacterium tuberculosis. In: Bennett J. E., Dolin R., Blaser M. J., editors. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases. 8th. Philadelphia, PA, USA: Elsevier; 2015. pp. 2787–2818.
    1. Jones B. E., Young S. M. M., Antoniskis D., Davidson P. T., Kramer F., Barnes P. F. Relationship of the manifestations of tuberculosis to CD4 cell counts in patients with human immunodeficiency virus infection. American Review of Respiratory Disease. 1993;148(5):1292–1297. doi: 10.1164/ajrccm/148.5.1292. - DOI - PubMed
    1. Yoon H. J., Song Y. G., Park W. I., Choi J. P., Chang K. H., Kim J. M. Clinical manifestations and diagnosis of extrapulmonary tuberculosis. Yonsei Medical Journal. 2004;45(3):p. 453. doi: 10.3349/ymj.2004.45.3.453. - DOI - PubMed

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