Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

doi:10.1093/neuonc/noy128

. 2018 Nov 12;20(12):1682-1683.

doi: 10.1093/neuonc/noy128.

Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

Affiliations

¹ Institute for Medical Genetics and Pathology, University Hospital Basel, Basel, Switzerland.
² Institute of Human Genetics, University Hospital Bonn, Bonn, Germany.
³ Department of Neurosurgery, University Hospital Basel, Basel, Switzerland.
⁴ Charité?Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Berlin, Germany.

PMID: 30169880
PMCID: PMC6231198
DOI: 10.1093/neuonc/noy128

Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

Jürgen Hench et al. Neuro Oncol. 2018.

. 2018 Nov 12;20(12):1682-1683.

doi: 10.1093/neuonc/noy128.

Authors

Affiliations

¹ Institute for Medical Genetics and Pathology, University Hospital Basel, Basel, Switzerland.
² Institute of Human Genetics, University Hospital Bonn, Bonn, Germany.
³ Department of Neurosurgery, University Hospital Basel, Basel, Switzerland.
⁴ Charité?Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Berlin, Germany.

PMID: 30169880
PMCID: PMC6231198
DOI: 10.1093/neuonc/noy128

No abstract available

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Figures

**Fig. 1**
(A) A cohort of 51 consecutive routine diagnostic samples examined with the fast tests (MLPA/PyroMark) and verified by Brain Tumor Methylation Classifier, delivering tumor type, CNVs, and MGMT promoter methylation status. (B) Comparison of MGMT promoter methylation values as determined by PyroMark and EPIC methylome array. Both techniques provide sufficient stratification based on published diagnostic cutoffs.^, (C–E) Turnaround times (TaT) calculated from diagnostic reports and plotted as histograms, counting from the day of surgery. DNA extraction follows overnight proteinase K tissue dissection. Robotic MLPA requires 19 h 35 min walk-away and approximately 10 min hands-on time due to automated master mix preparation. PyroMark bisulfite sequencing requires an additional overnight procedure compared with robotic MLPA. For economic reasons, methylation profiling requires parallel analysis of 16 specimens per microarray; actual processing time is 3 working days.

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References

1. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, eds. WHO Classification of Tumours of the Central Nervous System. Revised 4th ed Lyon: International Agency for Research on Cancer; 2016.
1. Capper D, Jones DTW, Sill M, et al. . DNA methylation-based classification of central nervous system tumours. Nature. 2018;555(7697):469–474. - PMC - PubMed
1. Jeuken J, Cornelissen S, Boots-Sprenger S, Gijsen S, Wesseling P. Multiplex ligation-dependent probe amplification: a diagnostic tool for simultaneous identification of different genetic markers in glial tumors. J Mol Diagn. 2006;8(4):433–443. - PMC - PubMed
1. Zlobec I, Bihl MP, Foerster A, Rufle A, Terracciano L, Lugli A. Stratification and prognostic relevance of Jass’s molecular classification of colorectal cancer. Front Oncol. 2012;2:7. - PMC - PubMed
1. Shirahata M, Ono T, Stichel D, et al. . Novel, improved grading system(s) for IDH-mutant astrocytic gliomas. Acta Neuropathol. 2018;136(1):153–166. - PubMed

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[1] Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, eds. WHO Classification of Tumours of the Central Nervous System. Revised 4th ed Lyon: International Agency for Research on Cancer; 2016.

[2] Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, eds. WHO Classification of Tumours of the Central Nervous System. Revised 4th ed Lyon: International Agency for Research on Cancer; 2016.

[3] Capper D, Jones DTW, Sill M, et al. . DNA methylation-based classification of central nervous system tumours. Nature. 2018;555(7697):469–474. - PMC - PubMed

[4] Capper D, Jones DTW, Sill M, et al. . DNA methylation-based classification of central nervous system tumours. Nature. 2018;555(7697):469–474. - PMC - PubMed

[5] Jeuken J, Cornelissen S, Boots-Sprenger S, Gijsen S, Wesseling P. Multiplex ligation-dependent probe amplification: a diagnostic tool for simultaneous identification of different genetic markers in glial tumors. J Mol Diagn. 2006;8(4):433–443. - PMC - PubMed

[6] Jeuken J, Cornelissen S, Boots-Sprenger S, Gijsen S, Wesseling P. Multiplex ligation-dependent probe amplification: a diagnostic tool for simultaneous identification of different genetic markers in glial tumors. J Mol Diagn. 2006;8(4):433–443. - PMC - PubMed

[7] Zlobec I, Bihl MP, Foerster A, Rufle A, Terracciano L, Lugli A. Stratification and prognostic relevance of Jass’s molecular classification of colorectal cancer. Front Oncol. 2012;2:7. - PMC - PubMed

[8] Zlobec I, Bihl MP, Foerster A, Rufle A, Terracciano L, Lugli A. Stratification and prognostic relevance of Jass’s molecular classification of colorectal cancer. Front Oncol. 2012;2:7. - PMC - PubMed

[9] Shirahata M, Ono T, Stichel D, et al. . Novel, improved grading system(s) for IDH-mutant astrocytic gliomas. Acta Neuropathol. 2018;136(1):153–166. - PubMed

[10] Shirahata M, Ono T, Stichel D, et al. . Novel, improved grading system(s) for IDH-mutant astrocytic gliomas. Acta Neuropathol. 2018;136(1):153–166. - PubMed

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Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

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Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

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