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. 2018 Nov 12;20(12):1682-1683.
doi: 10.1093/neuonc/noy128.

Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

Jürgen Hench  1 , Michel Bihl  1 , Ivana Bratic Hench  1 , Per Hoffmann  1   2 , Markus Tolnay  1 , Nemya Bösch Al Jadooa  1 , Luigi Mariani  1   3 , David Capper  4 , Stephan Frank  1
Affiliations

Satisfying your neuro-oncologist: a fast approach to routine molecular glioma diagnostics

Jürgen Hench et al. Neuro Oncol. .
No abstract available

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Figures

Fig. 1
Fig. 1
(A) A cohort of 51 consecutive routine diagnostic samples examined with the fast tests (MLPA/PyroMark) and verified by Brain Tumor Methylation Classifier, delivering tumor type, CNVs, and MGMT promoter methylation status. (B) Comparison of MGMT promoter methylation values as determined by PyroMark and EPIC methylome array. Both techniques provide sufficient stratification based on published diagnostic cutoffs., (C–E) Turnaround times (TaT) calculated from diagnostic reports and plotted as histograms, counting from the day of surgery. DNA extraction follows overnight proteinase K tissue dissection. Robotic MLPA requires 19 h 35 min walk-away and approximately 10 min hands-on time due to automated master mix preparation. PyroMark bisulfite sequencing requires an additional overnight procedure compared with robotic MLPA. For economic reasons, methylation profiling requires parallel analysis of 16 specimens per microarray; actual processing time is 3 working days.
See this image and copyright information in PMC

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