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. 2019 Jun;23(2):673-678.
doi: 10.1007/s11325-018-1713-8. Epub 2018 Aug 31.

Fas-positive lymphocytes are associated with systemic inflammation in obstructive sleep apnea syndrome

Joanna Domagała-Kulawik  1 , Iwona Kwiecień  2 , Piotr Bielicki  1 , Tomasz Skirecki  3
Affiliations

Fas-positive lymphocytes are associated with systemic inflammation in obstructive sleep apnea syndrome

Joanna Domagała-Kulawik et al. Sleep Breath. 2019 Jun.

Abstract

Purpose: Obstructive sleep apnea syndrome (OSAS) is associated with alterations in immune system which may lead to serious complications. The aim of this study was to explore lymphocyte populations in OSAS with special attention to the Fas-positive cells.

Methods: Fifty-one patients with confirmed OSA and 20 healthy subjects were investigated. The OSA severity indices, data concerning comorbidities, and markers of inflammation and metabolic disorders were collected. Flow cytometry was used to analyze the lymphocyte profile and expression of Fas receptors (CD95). Concentration of adiponectin, IL-1β, TNF-α, and sFas were measured.

Results: Proportions of Fas-positive cells in the pool of CD4+ and Fas-positive in the pool of CD8+ cells in the blood of patients were significantly increased when compared with healthy subjects (74.5% vs. 65.6% and 78.8% vs.70.9%, respectively, p < 0.05). No correlation with OSA severity was found. However, the proportion and number of Fas+ cells were elevated in obese patients, in non-smokers, and in patients suffering from COPD and hypertension. There were several significant relations of Fas+ cells with inflammatory markers of systemic inflammation.

Conclusion: Lymphocytes with the expression of Fas receptor are associated with systemic inflammation in OSAS.

Keywords: Fas receptor; Lymphocytes; OSAS; Systemic inflammation.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Representative flow cytometry analysis of Fas expression in blood lymphocytes in OSA patient. a Morphological gate for lymphocytes is shown, b then CD4+ and CD8+ cells are gated. Analysis of Fas (CD95) expression on c CD4+ and d CD8+ T cells
Fig. 2
Fig. 2
Scatter plots of correlations between Fas-positive T cells and other variables. a Count of CD4+Fas+ lymphocytes and polymorphonuclear cells (PMN) percentage. b Count of CD8+Fas+ lymphocytes and PMN percentage. c Proportion of CD4+Fas+ cell and NK cell percentage. d Proportion of CD8+Fas+ lymphocytes and NKT cell percentage. e Counts of CD4+Fas+ cells and NK cells. f Geometric mean fluorescence (GMF) of Fas receptor staining on CD4+ cells and oxygen saturation (SpO2). Dashed lines show 95% confidence intervals
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