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. 2018 Jun;24(6):499-503.

[Resveratrol protects human sperm against cryopreservation-induced injury]

[Article in Chinese]
Shi-Jia Li  1 , Wei-Dong Su  1 , Li-Jun Qiu  1 , Xiong Wang  2 , Juan Liu  3
Affiliations
  • PMID: 30173453

[Resveratrol protects human sperm against cryopreservation-induced injury]

[Article in Chinese]
Shi-Jia Li et al. Zhonghua Nan Ke Xue. 2018 Jun.

Abstract
in English, Chinese

Objective: To investigate the effects of resveratrol in the cryopreservation medium on the quality and function of post-thaw sperm.

Methods: Semen samples were obtained from 50 normozoospermic and 50 oligoasthenozoospermic men, liquefied and then cryopreserved in the glycerol-egg yolk-citrate (GEYC) medium with or without 30 μmol/L resveratrol. Sperm motility, viability and acrosome reaction (AR) were examined before and after thawing. Sperm lipid peroxidation and the level of reactive oxygen species (ROS) were measured using commercial malondialdehyde (MDA) and the ROS assay kit. Sperm mitochondrial membrane potential (MMP) and DNA damage were determined by Rhodamine 123 staining and TUNEL.

Results: The percentage of progressively motile sperm (PMS), total sperm motility, sperm viability, MMP and AR were significantly decreased (P <0.05) while the levels of sperm ROS, MDA and DNA fragmentation index (DFI) remarkably increased in both the normozoospermia and oligoasthenozoospermia groups after cryopreservation as compared with those in the fresh ejaculate (P <0.05). In comparison with the non-resveratrol control, the post-thaw sperm cryopreserved with 30 μmol/L resveratrol showed markedly higher PMS (［32.7 ± 4.8］ vs ［43.1 ± 6.3］ %, P <0.05), total motility (［44.8 ± 6.9］ vs ［56.9 ± 7.4］ %, P <0.05), viability (［52.3 ± 6.1］ vs ［67.5 ± 5.6］ %, P <0.05), MMP (［56.5 ± 7.0］ vs ［63.4 ± 7.5］ %, P <0.05) and AR (［16.6 ± 3.8］ vs ［26.3 ± 4.7］ %, P <0.05) but lower ROS, MDA and DFI (all P <0.05) in the normozoospermia group, and so did the post-thaw sperm in the oligoasthenozoospermia group, with a particularly lower DFI (［28.5 ± 4.8］ vs ［36.3 ± 5.7］%, P <0.01).

Conclusions: Resveratrol in the cryopreservation medium can improve the quality and function of post-thaw human sperm by reducing cryopreservation-induced sperm injury and the level of ROS.

目的： 通过在人精子冷冻保护液中添加白藜芦醇，研究其对冻融后精子质量和功能的影响。 方法： 选择正常精液与少弱精子症样本各50例，液化后的精液样本分别与甘油-卵黄-柠檬酸盐(GEYC)冷冻保护液或含有30 μmol/L白藜芦醇的GEYC冷冻保护液混匀。冷冻复苏前后，进行精子活力、存活率及顶体反应分析。采用丙二醛(MDA)及活性氧(ROS)检测试剂盒评估精子脂质过氧化程度及ROS水平。通过罗丹明123 (Rh123)染色法及TUNEL试验检测精子线粒体膜电位及DNA损伤。 结果： 在正常精液和少弱精子症样本组中，与各组冷 冻前新鲜精液相比，冻融后前向运动精子百分率、总活力、存活率、线粒体膜电位及顶体反应率均显著下降(P <0.05) ，而精子ROS、 MDA水平和DNA碎片指数(DFI)均显著升高 (P <0.05)。冷冻保护液中添加30 μmol/L白藜芦醇后，正常精液组前向运动精子百分率［(43.1 ± 6.3)%］、总活力［(56.9 ± 7.4)%］、存活率 ［(67.5 ± 5.6)%］、线粒体膜电位［(63.4 ± 7.5)%］及顶体反应百分率 ［(26.3 ± 4.7)%］ 较冷冻对照 (未加白藜芦醇)的前向运动精子百分率 ［(32.7 ± 4.8)%］ 、总活力［(44.8 ± 6.9)%］、存活率［(52.3 ± 6.1)%］、线粒体膜电位 ［(56.5 ± 7.0)%］ 及顶体反应百分率［(16.6 ± 3.8)%］均显著提高 (P <0.05)，而精子ROS、 MDA水平和DFI较冷冻对照均显著降低 (P <0.05)。在少弱精子症组中，添加白藜芦醇也均显著地提高了冷冻后精子前向运动百分率、总活力百分率、存活率、线粒体膜电位及顶体反应百分率，尤其是DFI ［28.5 ± 4.8)%］较冷冻对照［(36.3 ± 5.7)%］显著降低 (P<0.01)。 结论： 在精液冷冻保护液中添加白藜芦醇可以通过降低精子内ROS水平减少精子冷冻损伤，从而改善解冻后精子质量和功能。.

Keywords: cryopreservation; oxidative stress injury; sperm; sperm function; sperm quality; resveratrol.

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