A routine method for the establishment of permanent growing lymphoblastoid cell lines
- PMID: 3017841
- DOI: 10.1007/BF00279094
A routine method for the establishment of permanent growing lymphoblastoid cell lines
Abstract
Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.
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