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. 1986 Aug;73(4):320-6.
doi: 10.1007/BF00279094.

A routine method for the establishment of permanent growing lymphoblastoid cell lines

A routine method for the establishment of permanent growing lymphoblastoid cell lines

H Neitzel. Hum Genet. 1986 Aug.

Abstract

Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.

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