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. 1986 Sep;167(3):1086-8.
doi: 10.1128/jb.167.3.1086-1088.1986.

Genetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci

Genetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci

S Tirgari et al. J Bacteriol. 1986 Sep.

Abstract

The nadA and pnuC loci of S. typhimurium were cloned and found to reside within a 2.2-kilobase region. Two-dimensional O'Farrell gel electrophoresis of the proteins produced after chloramphenicol amplification and subsequent release from chloramphenicol inhibition revealed NadA and PnuC to be 43,000- and 25,000-molecular-weight proteins, respectively. The data indicated that nadA and pnuC represent two distinct genes.

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