Genetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci
- PMID: 3017917
- PMCID: PMC215988
- DOI: 10.1128/jb.167.3.1086-1088.1986
Genetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci
Abstract
The nadA and pnuC loci of S. typhimurium were cloned and found to reside within a 2.2-kilobase region. Two-dimensional O'Farrell gel electrophoresis of the proteins produced after chloramphenicol amplification and subsequent release from chloramphenicol inhibition revealed NadA and PnuC to be 43,000- and 25,000-molecular-weight proteins, respectively. The data indicated that nadA and pnuC represent two distinct genes.
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