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Comparative Study
. 1986 Aug;18(4):307-14.
doi: 10.1007/BF00743051.

Isolation and characterization of human heart cytochrome c oxidase

Comparative Study

Isolation and characterization of human heart cytochrome c oxidase

L Kuhn-Nentwig et al. J Bioenerg Biomembr. 1986 Aug.

Abstract

Cytochrome c oxidase was isolated from human hearts and separated by SDS gel electrophoresis. The identity of polypeptide bands with known subunits was demonstrated by immunoblotting with monospecific antisera to rat liver cytochrome c oxidase subunits. The polarographically determined kinetics of cytochrome c oxidation were similar to those reported for the bovine heart enzyme.

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