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. 2018 Oct 24;62(11):e00374-18.
doi: 10.1128/AAC.00374-18. Print 2018 Nov.

Absence of a High Level of Duplication of the Plasmepsin II Gene in Africa

Marie Gladys Robert  1   2 , Francis Foguim Tsombeng  1   2 , Mathieu Gendrot  1   2 , Joel Mosnier  1   2   3 , Rémy Amalvict  1   2   3 , Nicolas Benoit  1   2   3 , Marylin Torrentino-Madamet  1   2   3 , Bruno Pradines  4   2   3 ; French National Reference Centre for Imported Malaria Study Group
Collaborators, Affiliations

Absence of a High Level of Duplication of the Plasmepsin II Gene in Africa

Marie Gladys Robert et al. Antimicrob Agents Chemother. .

Abstract

Resistance to piperaquine has been associated with the amplification of the plasmepsin II gene in Cambodia. None of the 175 African isolates that we analyzed had plasmepsin II gene amplification (piperaquine 50% inhibitory concentration ranged from 0.94 to 137.5 nM), suggesting there is a low prevalence of piperaquine reduced susceptibility in Africa. Additionally, the few isolates with reduced susceptibility to piperaquine did not harbor amplification of the plasmepsin II gene.

Keywords: Plasmodium falciparum; antimalarial drug; in vitro; malaria; molecular marker; piperaquine; plasmepsin II gene; resistance.

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Figures

FIG 1
FIG 1
Distribution of ex vivo responses (IC50) of 175 African Plasmodium falciparum isolates to piperaquine.
FIG 2
FIG 2
Copy number of the plasmepsin II gene as a function of piperaquine IC50 of 175 African Plasmodium falciparum isolates.
See this image and copyright information in PMC

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