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. 1986 Jul;204(1):1-7.
doi: 10.1007/BF00330179.

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

Y Ishino, H Shinagawa, K Makino, S Tsunasawa, F Sakiyama, A Nakata

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

Y Ishino et al. Mol Gen Genet. 1986 Jul.

Abstract

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

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