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Comparative Study
. 1986 Aug 11;14(15):6017-30.
doi: 10.1093/nar/14.15.6017.

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Y Xia, D E Burbank, J L Van Etten
Free PMC article
Comparative Study

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Y Xia et al. Nucleic Acids Res. .
Free PMC article

Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

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