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. 2018 Aug 31;4(8):e00766.
doi: 10.1016/j.heliyon.2018.e00766. eCollection 2018 Aug.

Chiral zirconium quantum dots: A new class of nanocrystals for optical detection of coronavirus

Syed Rahin Ahmed  1 , Seon Woo Kang  2 , Sangjin Oh  2 , Jaebeom Lee  2 , Suresh Neethirajan  1
Affiliations

Chiral zirconium quantum dots: A new class of nanocrystals for optical detection of coronavirus

Syed Rahin Ahmed et al. Heliyon. .

Abstract

A synthetic way of chiral zirconium quantum dots (Zr QDs) was presented for the first time using L(+)-ascorbic acid acts as a surface as well as chiral ligands. Different spectroscopic and microscopic analysis was performed for thorough characterization of Zr QDs. As-synthesized QDs exhibited fluorescence and circular dichroism properties, and the peaks were located at 412 nm and 352 nm, respectively. MTT assay was performed to test the cytotoxicity of the synthesized Zr QDs against rat brain glioma C6 cells. Synthesized QDs was further conjugated with anti-infectious bronchitis virus (IBV) antibodies of coronavirus to form an immunolink at the presence of the target analyte and anti-IBV antibody-conjugated magneto-plasmonic nanoparticles (MPNPs). The fluorescence properties of immuno-conjugated QD-MP NPs nanohybrids through separation by an external magnetic field enabled biosensing of coronavirus with a limit of detection of 79.15 EID/50 μL.

Keywords: Biomedical engineering; Materials chemistry; Materials science; Nanotechnology.

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Figures

Fig. 1
Fig. 1
Scheme of sensor design: (A) Zr nanoparticles and reducing agent keep on vial; (B) Zr QDs formation; (C) antibody conjugated QDs; (D) the addition of antibody-conjugated MP NPs; (E) formation of nanostructured magnetoplasmonic-fluorescent with the addition of target, then separated (F); (G) the nanohybrid-conjugated part was dispersed and measure the optical properties (H).
Fig. 2
Fig. 2
Optical properties of Zr QDs: (A) PL intensity and absorbance spectra of Zr QDs (B); (C) chiral spectra and (D) color of solution.
Fig. 3
Fig. 3
Image of Zr QDs and Zr NPs: (A) Scheme of Zr QD synthesis; (B) HRTEM image of ZrNPs and QDs (C) (inset: size distribution profile); (D) HRTEM image of Zr QDs (inset: lattice pattern of ZrQDs).
Fig. 4
Fig. 4
XPS data of Zr NPs (A) and Zr QDs (B).
Fig. 5
Fig. 5
X-ray diffraction (XRD) spectra of synthesized Zr QDs.
Fig. 6
Fig. 6
Cell viability study of Zr QDs.
Fig. 7
Fig. 7
Characterization of MP NPs: (A) Large-scale TEM image of MP NPs; (B) close-up view of MP NPs (inset: color of solution); (C) the absorbance spectra of MP NPs; and (D) the SQUID measurement of MP NPs.
Fig. 8
Fig. 8
The specificity of anti-IBV antibodies for coronavirus.
Fig. 9
Fig. 9
The conjugation of antibodies with nanomaterials.
Fig. 10
Fig. 10
FTIR spectra of MP NPs and anti-IBV antibodies binding.
Fig. 11
Fig. 11
Fluorescence-based detection of IBV: (A) PL intensity responses; (B) Target virus concentration dependent PL intensity; (C) PL intensity Vs virus concentration curve; and (D) selectivity test.
See this image and copyright information in PMC

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