Herpes simplex virus type 1 persistence and latency in cultured rabbit corneal epithelial cells, keratocytes, and endothelial cells

Abstract

Cell cultures of rabbit corneal epithelium, keratocytes, and endothelium were used to determine the lytic cycle of herpes simplex virus type 1. Viral growth was fastest in epithelial cells. A novel HSV-1 in-vitro latency system was established in the three distinct cell types. Cell cultures were inoculated at low multiplicities of infection with HSV-1. Temperature manipulation alone was used to induce and reactivate latent HSV-1 infections. The presence of cellular stress proteins was demonstrated at supraoptimal temperatures. All cell types were capable of maintaining latent viral infections under these conditions. Viral persistence was present in 20% of epithelial cell cultures at supraoptimal temperatures, but not in keratocyte cultures or endothelial cell cultures.