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Comment
. 2018 Sep;178(1):11-12.
doi: 10.1104/pp.18.00969.

An Improved Tool for Mapping the Membrane-Associated Protein Interactome

Chunhua Zhang  1
Affiliations
Comment

An Improved Tool for Mapping the Membrane-Associated Protein Interactome

Chunhua Zhang. Plant Physiol. 2018 Sep.
No abstract available

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Figures

Figure 1.
Figure 1.
Traditional mating-based split-ubiquitin system and GPI signal peptide-anchored split-ubiquitin system. A, In mating-based split-ubiquitin systems, when membrane-associated bait and prey proteins do not interact, the split ubiquitin cannot fold properly to form functional ubiquitin and cannot be recognized by USPs. PLV is not released and the reporter genes are not activated. B, In mating-based split-ubiquitin systems, when membrane-associated bait and prey proteins interact with each other, the Nub and Cub form functional ubiquitin, which is recognized by USPs. PLV is released and then diffuses to the nucleus to activate reporter genes. C, In GPS, a GPI signal peptide is added to the N terminus of the bait protein to increase the efficiency of membrane targeting. Bait proteins thus are localized to the membrane even when they do not normally have a membrane-anchoring sequence. It is not clear how this signal peptide serves as a membrane anchor. The prey protein can be soluble protein in all cases shown.
See this image and copyright information in PMC

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References

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