Proteomics of Anopheles Vectors of Malaria

Abstract

Proteomic investigations in Anopheles gained momentum following the sequencing of the Anopheles gambiae genome, allowing peptide data from mass spectrometry to be searched against large datasets of predicted protein sequences. Exhaustive discovery proteomics investigations have improved the annotation of genomic datasets and catalogued proteins from mosquito tissues, including the salivary glands, midgut, and sensory appendages. These efforts have revealed protein constituents that define the unique biological functions of these organs. Quantitative proteomics investigations have begun to characterise the molecular basis of mosquito behaviour and immune responses. With a current trend towards increasing sensitivity of mass spectrometers and simpler workflows, proteomics is set to accelerate the development of antiparasite interventions through the identification of new targets for parasite or vector control and diagnostic biomarkers.

Keywords: Anopheles; mass spectrometry; mosquito vectors; proteome; tissue analysis.