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. 1986 Aug;5(8):1959-66.
doi: 10.1002/j.1460-2075.1986.tb04450.x.

Identification of an Epstein-Barr virus-coded thymidine kinase

E Littler, J Zeuthen, A A McBride, E Trøst Sørensen, K L Powell, J E Walsh-Arrand, J R Arrand

Identification of an Epstein-Barr virus-coded thymidine kinase

E Littler et al. EMBO J. 1986 Aug.

Abstract

We have demonstrated the presence of an Epstein-Barr virus (EBV)-coded thymidine kinase (TK) by producing biochemically transformed, TK-positive mammalian cell lines using either microinjection of whole EBV virions or calcium phosphate-mediated transfection of the SalI-B restriction endonuclease fragment of EBV DNA. Analysis of these cell lines showed that: (i) EBV DNA was present in the cell lines, (ii) sequences from the SalI-B restriction endonuclease fragment of EBV were expressed, (iii) a TK activity was present and (iv) a protein with antigenic cross-reactivity with the herpes simplex virus (HSV) TK was produced. The identity of the EBV TK gene was determined by demonstrating that a recombinant plasmid, which expressed the protein product of the BXLF1 open reading frame as a fusion protein, could complement TK- strains of E. coli. A comparison of the predicted amino acid sequences of the TK proteins of EBV and HSV-1 revealed significant regions of homology.

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References

    1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):433-6 - PubMed
    1. J Clin Pathol. 1965 May;18:261-73 - PubMed
    1. J Biol Chem. 1980 Jun 10;255(11):5120-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 - PubMed
    1. Virology. 1980 Jul 15;104(1):219-23 - PubMed

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