Investigation of GLUT1, HIF1α and TBX21 Gene Polymorphisms in Laryngeal Cancer

Abstract

Objective: We aimed to investigate the association of the frequency of three single-nucleotide polymorphisms [glucose transporter isoform 1 (GLUT1) reference single-nucleotide polymorphism (rs) 710218, hypoxia-inducible factor 1 alpha (HIF1α) rs11549465, and T-box transcription factor protein 21 (TBX21) rs17250932], which have been proved to be related with various benign and malignant diseases, with the development of laryngeal cancer and its size and grade.

Methods: In this study, we included 35 patients with laryngeal cancer and 35 volunteers at least 30 years old who had smoked for at least 20 years. DNA was obtained from the blood samples of the participants using an isolation kit. Then, polymorphisms for both the groups were determined using real-time polymerase chain reaction.

Results: No significant differences were detected regarding the genotype and allele frequencies in the three polymorphisms assessed between the two groups. In the patient group, on examining the association of polymorphisms with tumor size and grade, no significant relation was observed in three polymorphisms regarding the related parameters.

Conclusion: GLUT1, HIF1α, and TBX21 polymorphisms have no impact on the development of laryngeal cancer.

Keywords: Cancer; GLUT1; HIF1α; TBX21; larynx; polymorphism.

Figure 1

GLUT1 rs710218 study group genotype distribution (A: Adenine, T: Thymine)

Figure 2

GLUT1 rs710218 control group genotype distribution (A: Adenine, T: Thymine)

Figure 3

HIF1α rs11549465 study group genotype distribution (C: Cytosine, T: Thymine)

Figure 4

HIF1α rs11549465 control group genotype distribution (C: Cytosine, T: Thymine)

Figure 5

TBX21 rs17250932 study group genotype distribution (C: Cytosine, T: Thymine)

Figure 6

TBX21 rs17250932 control group genotype distribution (C: Cytosine, T: Thymine)