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. 2018 Aug 30;23(9):2190.
doi: 10.3390/molecules23092190.

Biofilm Inhibitory Abscisic Acid Derivatives from the Plant-Associated Dothideomycete Fungus, Roussoella sp

Chayanard Phukhamsakda  1 , Allan Patrick G Macabeo  2   3 , Kamila Tomoko Yuyama  4 , Kevin David Hyde  5 , Marc Stadler  6
Affiliations

Biofilm Inhibitory Abscisic Acid Derivatives from the Plant-Associated Dothideomycete Fungus, Roussoella sp

Chayanard Phukhamsakda et al. Molecules. .

Abstract

Roussoella species are well recorded from both monocotyledons and dicotyledons. As part of a research program to discover biologically active compounds from plant-associated Dothideomycetes in Thailand, the strain Roussoella sp. (MFLUCC 17-2059), which represents an undescribed species, was isolated from Clematis subumbellata Kurz, fermented in yeast-malt medium and explored for its secondary metabolite production. Bioassay-guided fractionation of the crude extract yielded the new abscisic acid derivative, roussoellenic acid (1), along with pestabacillin B (2), a related congener, and the cyclodipeptide, cyclo(S-Pro-S-Ile) (3). The structure of 1 was determined by 2D NMR spectroscopy and HR-ESIMS data analysis. Compounds 1 and 2 showed inhibitory activity on biofilm formation by Staphylococcus aureus. The biofilm formation of S. aureus was reduced to 34% at 16 µg/mL by roussoellenic acid (1), while pestabacillin B (2) only showed 36% inhibition at 256 µg/mL. In addition, compound 1 also had weak cytotoxic effects on L929 murine fibroblasts and human KB3-1 cancer cells.

Keywords: Anti-biofilm; Ascomycota; Pleosporales; structure elucidation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Abscisic acid derivatives and cyclodipeptide from Roussoella sp. MFLUCC 17-2059.
Figure 2
Figure 2
COSY and key HMBC correlations in 1.
Scheme 1
Scheme 1
Putative biogenetic pathway of 1 and 2.
See this image and copyright information in PMC

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