Antibiotic-Induced Genetic Variation: How It Arises and How It Can Be Prevented

Abstract

By targeting essential cellular processes, antibiotics provoke metabolic perturbations and induce stress responses and genetic variation in bacteria. Here we review current knowledge of the mechanisms by which these molecules generate genetic instability. They include production of reactive oxygen species, as well as induction of the stress response regulons, which lead to enhancement of mutation and recombination rates and modulation of horizontal gene transfer. All these phenomena influence the evolution and spread of antibiotic resistance. The use of strategies to stop or decrease the generation of resistant variants is also discussed.

Keywords: ROS; SOS; antibiotic resistance; evolvability; genetic instability; mutagenesis; mutation rates.