Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections
- PMID: 3020087
- PMCID: PMC268946
- DOI: 10.1128/jcm.24.3.487-489.1986
Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections
Abstract
The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.
Similar articles
-
Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen.J Clin Microbiol. 1984 Jun;19(6):730-2. doi: 10.1128/jcm.19.6.730-732.1984. J Clin Microbiol. 1984. PMID: 6088568 Free PMC article.
-
Comparison of the Ortho Cultureset (Vero cells) and Difco Cellmatics system (mink lung and primary rabbit kidney cells) for the detection of herpes simplex virus from clinical specimens.Diagn Microbiol Infect Dis. 1987 Feb;6(2):145-9. doi: 10.1016/0732-8893(87)90099-x. Diagn Microbiol Infect Dis. 1987. PMID: 3028705
-
Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus.J Clin Microbiol. 1984 Jun;19(6):920-2. doi: 10.1128/jcm.19.6.920-922.1984. J Clin Microbiol. 1984. PMID: 6088575 Free PMC article.
-
Laboratory diagnosis of herpes simplex virus type 1 and type 2 infections.Clin Dermatol. 1984 Apr-Jun;2(2):67-82. doi: 10.1016/0738-081x(84)90067-1. Clin Dermatol. 1984. PMID: 6100717 Review. No abstract available.
-
Diagnosis of herpes simplex virus infections of the CNS.Expert Rev Mol Diagn. 2005 Jul;5(4):537-47. doi: 10.1586/14737159.5.4.537. Expert Rev Mol Diagn. 2005. PMID: 16013972 Review.
Cited by
-
Continuous high-speed rolling versus centrifugation for detection of herpes simplex virus.J Clin Microbiol. 1989 Dec;27(12):2884-6. doi: 10.1128/jcm.27.12.2884-2886.1989. J Clin Microbiol. 1989. PMID: 2556440 Free PMC article.
-
Laboratory techniques in the diagnosis of herpes simplex infection.Genitourin Med. 1993 Jun;69(3):174-83. doi: 10.1136/sti.69.3.174. Genitourin Med. 1993. PMID: 8392966 Free PMC article. Review. No abstract available.
-
Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.J Clin Microbiol. 1987 Aug;25(8):1401-5. doi: 10.1128/jcm.25.8.1401-1405.1987. J Clin Microbiol. 1987. PMID: 3040800 Free PMC article.
-
Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.J Clin Microbiol. 1991 Mar;29(3):650-2. doi: 10.1128/jcm.29.3.650-652.1991. J Clin Microbiol. 1991. PMID: 1645373 Free PMC article.
-
Physical and chemical methods for enhancing rapid detection of viruses and other agents.Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150. Clin Microbiol Rev. 1993. PMID: 8472247 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical