Influence of apheresis collection device and container on the storage properties of platelets in 90% PAS-5/10% plasma

Abstract

Background: The storage properties of apheresis platelets suspended in the experimental additive solution PAS-5 and 10% plasma may be affected by the collection instrument or storage container.

Methods and experimental design: The same consenting 12 donors provided A or T platelets with concurrent plasma on four occasions in 100% plasma. Following collection and resting, the platelets were centrifuged, and plasma was expressed and resuspended in PAS-5 to yield units with 10% plasma. Platelets were either maintained in the original storage container or transferred to another of the manufacturer's storage containers. On days 1, 5 and 7, units were assayed for an array of in vitro tests.

Results: Average unit volume, yield and percent plasma was 291±11 mL, 3.7±0.4×10¹¹, and 10.3±0.7%, respectively, and were comparable between collections with either of the apheresis instruments and stored with either of the manufacturer's containers. Day 1 platelet activation (CD62P+) was 40±22% and was similar in either of the collection instruments or containers. Except for pH (days 1, 5), CO₂ (days 1, 5, 7), and extent of shape change (day 5), every other in vitro parameter was similar between apheresis platforms or the manufacturer's container. pH values of all units on all days of storage were ≥6.8, except one unit that was collected on T and stored in an A container, which had pH values of 6.8 and 5.7 on days 5 and 7, respectively.

Discussion: Storage of platelets suspended in PAS-5 with 10% plasma is feasible in the original manufacturer's container for seven days. Based on CO₂ levels, T containers have greater gas exchange than A containers.