iRNA(m6A)-PseDNC: Identifying N6-methyladenosine sites using pseudo dinucleotide composition

Abstract

As a prevalent post-transcriptional modification, N⁶-methyladenosine (m⁶A) plays key roles in a series of biological processes. Although experimental technologies have been developed and applied to identify m⁶A sites, they are still cost-ineffective for transcriptome-wide detections of m⁶A. As good complements to the experimental techniques, some computational methods have been proposed to identify m⁶A sites. However, their performance remains unsatisfactory. In this study, we firstly proposed an Euclidean distance based method to construct a high quality benchmark dataset. By encoding the RNA sequences using pseudo nucleotide composition, a new predictor called iRNA(m6A)-PseDNC was developed to identify m⁶A sites in the Saccharomyces cerevisiae genome. It has been demonstrated by the 10-fold cross validation test that the performance of iRNA(m6A)-PseDNC is superior to the existing methods. Meanwhile, for the convenience of most experimental scientists, established at the site http://lin-group.cn/server/iRNA(m6A)-PseDNC.php is its web-server, by which users can easily get their desired results without need to go through the detailed mathematics. It is anticipated that iRNA(m6A)-PseDNC will become a useful high throughput tool for identifying m⁶A sites in the S. cerevisiae genome.

Keywords: 5-step rules; N(6)-methyladenosine; Pseudo nucleotide composition; RNA modification; Support vector machine.