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. 2018 Sep;26(6):786-789.
doi: 10.1016/j.jsps.2018.04.005. Epub 2018 Apr 3.

Vitamin B12 protects against DNA damage induced by hydrochlorothiazide

Karem H Alzoubi  1 , Erva Bayraktar  1 , Omar Khabour  2 , Sayer I Al-Azzam  1
Affiliations

Vitamin B12 protects against DNA damage induced by hydrochlorothiazide

Karem H Alzoubi et al. Saudi Pharm J. 2018 Sep.

Abstract

DNA damage induced by hydrochlorothiazide was previously reported in cultured human lymphocytes. In this study, we aimed to investigate the harmful effects of hydrochlorothiazide on DNA by measuring the spontaneous frequency of sister chromatid exchanges (SCEs) in cultured human lymphocytes. We also aimed to investigate the possible protection of that damage by vitamin B12. The results showed that hydrochlorothiazide (5 µg/mL) significantly increased the frequency of sister chromatid exchanges (P < 0.001) in human lymphocytes in comparison with control. Additionally, the frequency of hydrochlorothiazide-induced SCEs was significantly decreased by co-treatment with vitamin B12 at concentration of 13.5 µg/mL (P < 0.001). In conclusion, hydrochlorothiazide is genotoxic to human lymphocytes and its toxicity is reduced by vitamin B12.

Keywords: DNA damage; Genotoxicity; Hydrochlorothiazide; Sister chromatids exchange; Vitamin B12.

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Figures

Fig. 1
Fig. 1
The average of SCEs/cell in control, Vit. B12, hydrochlorothiazide, and Vit. B12+HCT groups. P (P < 0.001) using Tukey's multiple comparison Test. * Indicates significant difference from control and Vit. B12. $ Indicates significant difference from HCT group.
Fig. 2
Fig. 2
The values of mitotic indices in control, Vit. B12, HCT and Vit. B12+HCT groups. P > 0.05 using Tukey's multiple comparison Test.
Fig. 3
Fig. 3
The proliferative indices of blood lymphocytes in control, Vit. B12, HCT and Vit. B12+HCT groups. P > 0.05 using Tukey's multiple comparison Test.
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