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. 2018 Sep 4:11:51.
doi: 10.1186/s13039-018-0399-8. eCollection 2018.

Chromosomes of Asian cyprinid fishes: cytogenetic analysis of two representatives of small paleotetraploid tribe Probarbini

Pasakorn Saenjundaeng  1 , Marcelo de Bello Cioffi  2 , Ezequiel Aguiar de Oliveira  2   3 , Alongklod Tanomtong  1 , Weerayuth Supiwong  4 , Sumalee Phimphan  1 , Maria João Collares-Pereira  5 , Alexandr Sember  6 , Luiz Antonio Carlos Bertollo  2 , Thomas Liehr  7 , Cassia Fernanda Yano  2 , Terumi Hatanaka  2 , Petr Ráb  6
Affiliations

Chromosomes of Asian cyprinid fishes: cytogenetic analysis of two representatives of small paleotetraploid tribe Probarbini

Pasakorn Saenjundaeng et al. Mol Cytogenet. .

Abstract

Background: Polyploidy, although still poorly explored, represents an important evolutionary event in several cyprinid clades. Herein, Catlocarpio siamensis and Probarbus jullieni - representatives of the paleotetraploid tribe Probarbini, were characterized both by conventional and molecular cytogenetic methods.

Results: Alike most other paleotetraploid cyprinids (with 2n = 100), both species studied here shared 2n = 98 but differed in karyotypes: C. siamensis displayed 18m + 34sm + 46st/a; NF = 150, while P. jullieni exhibited 26m + 14sm + 58st/a; NF = 138. Fluorescence in situ hybridization (FISH) with rDNA probes revealed two (5S) and eight (18S) signals in C. siamensis, respectively, and six signals for both probes in P. jullieni. FISH with microsatellite motifs evidenced substantial genomic divergence between both species. The almost doubled size of the chromosome pairs #1 in C. siamensis and #14 in P. jullieni compared to the rest of corresponding karyotypes indicated chromosomal fusions.

Conclusion: Based on our findings, together with likely the same reduced 2n = 98 karyotypes in the remainder Probarbini species, we hypothesize that the karyotype 2n = 98 might represent a derived character, shared by all members of the Probarbini clade. Besides, we also witnessed considerable changes in the amount and distribution of certain repetitive DNA classes, suggesting complex post-polyploidization processes in this small paleotetraploid tribe.

Keywords: Chromosomal markers; Cyprinidae; Fish cytogenetics; Microsatellites; rDNAs.

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Conflict of interest statement

Experiments were performed in accordance with ethical protocols, and anesthesia using clove oil was administered prior to sacrificing the animals, as approved by the Ethics Committee of Khon Kaen University and by the RGJ committee under no. PHD/K0081/2556.Not applicableThe authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Collection sites of Catlocarpio siamensis and Probarbus jullieni in Thailand examined in the present study (Map of the Mekong River basin modified from Rainboth [13])
Fig. 2
Fig. 2
Karyotypes of Catlocarpio siamensis and Probarbus jullieni arranged from Giemsa-stained, C-banded chromosomes and after dual-colour FISH with 5S (red) and 18S (green) rDNA probes. Scale bar = 5 μm
Fig. 3
Fig. 3
Metaphase plates of Catlocarpio siamensis and Probarbus jullieni after FISH with different microsatellite motifs. Scale bar = 5 μm
See this image and copyright information in PMC

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