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. 2018 Sep 3;10(1):1510712.
doi: 10.1080/20002297.2018.1510712. eCollection 2018.

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Talita Castro  1 , Maite Sabalza  2 , Cheryl Barber  2 , William Abrams  2 , Antonio Charlys Da Costa  3 , Flavio Augusto De Pádua Milagres  4 , Paulo Henrique Braz-Silva  1   3 , Daniel Malamud  2 , Marina Gallottini  1
Affiliations

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Talita Castro et al. J Oral Microbiol. .

Abstract

Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.

Keywords: Dengue; Flavivirus; LAMP; RNA; Zika; infection; polymerase chain reaction; saliva; virology.

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Figures

Figure 1.
Figure 1.
Number of positive samples of saliva and urine and their p-value (Chi-square test).
Figure 2.
Figure 2.
Time to positivity between saliva and urine samples with average and p-value (t-test).
See this image and copyright information in PMC

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