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. 2018 Nov;68(11):3557-3562.
doi: 10.1099/ijsem.0.003031. Epub 2018 Sep 11.

Mycobacterium decipiens sp. nov., a new species closely related to the Mycobacterium tuberculosis complex

Barbara A Brown-Elliott  1 , Patricia J Simmer  2 , Alberto Trovato  3 , Emily P Hyle  4 , Sara Droz  5 , Seanne P Buckwalter  6 , Emanuele Borroni  3 , John A Branda  7 , Elkina Iana  8 , Alessandro Mariottini  9 , Jameelah Nelson  10 , Alberto Matteelli  11 , Nadege C Toney  12 , Claudio Scarparo  13 , Tom J B de Man  12 , Ravikiran Vasireddy  1 , Rajesh T Gandhi  4 , Nancy L Wengenack  6 , Daniela M Cirillo  3 , Richard J Wallace  1 , Enrico Tortoli  3
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Mycobacterium decipiens sp. nov., a new species closely related to the Mycobacterium tuberculosis complex

Barbara A Brown-Elliott et al. Int J Syst Evol Microbiol. 2018 Nov.

Abstract

Two mycobacterial strains with close similarity to the Mycobacterium tuberculosis complex (MTBC) were isolated from cutaneous lesions of patients in the USA and Italy. At the phenotypic level, similarities to the MTBC included slow growth rate, rough morphotype of the unpigmented colonies and nearly identical high-performance liquid chromatography profiles of mycolic acids. In contrast to the MTBC, the strains were niacin- and nitrate-negative, and catalase-positive both at 68 °C and in semi-quantitative tests. The clinical isolates were more closely related to M. tuberculosis than to any other known mycobacterium and scored positive with commercial DNA probes (Hologic AccuProbe M. tuberculosis). Both average nucleotide identity and genome-to-genome distance suggested the strains are different from the MTBC. Therefore, given the distinguishing phenotypic and genomic-scale differences, we submit that the strains belong to a new species we have named Mycobacteriumdecipiens with type strain TBL 1200985T (=ATCC TSD-117T=DSM 105360T).

Keywords: Mycobacterium decipiens; Mycobacterium tuberculosis complex; average nucleotide identity; whole genome sequencing.

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Conflict of interest statement

Conflicts of interest

The authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Representative mycolic acid patterns of strain TBL 1 200985T and M. tuberculosis. LMMIS, low molecular mass internal standard; HMMIS, high molecular mass internal standard.
Fig. 2.
Fig. 2.
Phylogenetic tree based on 16S rRNA sequences of representative species of the genus Mycobacterium, reconstructed using the neighbour-joining method bootstrapped 1000 times. Bootstrap values >50 are given at nodes. Bar, 0.005 substitutions per nucleo-tide position.
Fig. 3.
Fig. 3.
Phylogenetic tree based on concatenated sequences of 16S rRNA, hsp65 and rpoB gene sequences of representative species of the genus Mycobacterium, reconstructed using the neighbour-joining method bootstrapped 1000 times. Bootstrap values >50 are given at nodes. Bar, 0.01 substitutions per nucleotide position.
See this image and copyright information in PMC

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